Molekulare Diagnostik
1. Originalarbeiten
Draft genome sequence of Bacillus anthracis UR-1 isolated from a
German heroin user
Christian Rückert, Katharina Licht, Joern Kalinowski, Christophe Espirito Santo, Markus Antwerpen, Matthias Hanczaruk, Udo Reischl, Thomas Holzmann, André Gessner, Carsten Tiemann and Gregor Grass
2012, Journal of Bacteriology
Draft genome sequence of Bacillus anthracis BF-1 isolated from Bavarian cattle
Markus Antwerpen, Diogo Proença, Christian Rueckert, Katharina Licht, Joern Kalinowski, Matthias Hanczaruk, Carsten Tiemann and Gregor Grass
2012, Journal of Bacteriology
H1N1, der Erreger des Jahres 2009 – Pandemie ohne Panik
Tiemann C, 2009, Trillium Report
A Cryptographic Biometric Authentication System based on Genetic Fingerprints.
Ulrike Korte, Michael Krawczak, Johannes Merkle, Rainer Plaga, Matthias Niesing, Carsten Tiemann, Han Vinck, Ulrich Martini:. Sicherheit 2008: 263-276
Frequency of very low HCV viremia detected by a highly sensitive HCV-RNA assay.
Fytili P, Tiemann C, Wang C, Schulz S, Schaffer S, Manns MP, Wedemeyer H.
J Clin Virol. 2007 Aug;39(4):308-11. Epub 2007 Jul 12.
Multicenter evaluation of the new Abbott RealTime assays for quantitative detection of human immunodeficiency virus type 1 and hepatitis C virus RNA.
Schutten M, Peters D, Back NK, Beld M, Beuselinck K, Foulongne V, Geretti AM, Pandiani L, Tiemann C, Niesters HG.
J Clin Microbiol. 2007 Jun;45(6):1712-7. Epub 2007 Apr 4.
Detection of Treponema pallidum in the vitreous by PCR.
Müller M, Ewert I, Hansmann F, Tiemann C, Hagedorn HJ, Solbach W, Roider J, Nölle B, Laqua H, Hoerauf H.
Br J Ophthalmol. 2007 May;91(5):592-5. Epub 2006 Nov 15.
Ultra-rapid detection of Chlamydia trachomatis by real-time PCR in the LightCycler using SYBR green technology or 5′-nuclease probes.
Eickhoff M, Laue T, Ruckes T, Cramer SO, Krupp G, Tiemann C.
Clin Lab. 2003;49(5-6):217-25.
Rapid DNA typing of HLA-B27 allele by real-time PCR using lightcycler technology.
Tiemann C, Vogel A, Dufaux B, Zimmer M, Krone JR, Hagedorn HJ.
Clin Lab. 2001;47(3-4):131-4.
Effect of cyclosporine A on the release of tissue factor pathway inhibitor from endothelial cells in heart transplant patients and cell culture.
Tiemann C, Prohaska W, Körfer R, Körner M, Brinkmann T, Kleesiek K.
Eur J Clin Chem Clin Biochem. 1997 Sep;35(9):661-7.
Detection of the three Kunitz-type single domains of membrane-bound tissue factor pathway inhibitor (TFPI) by flow cytometry.
Tiemann C, Brinkmann T, Kleesiek K.
Eur J Clin Chem Clin Biochem. 1997 Nov;35(11):855-60.
Genomic organization and sequence of the rat major histocompatibility complex class Ia gene RT1.Au.
Walter L, Tiemann C, Heine L, Günther E.
Immunogenetics. 1995;41(5):332. No abstract available.
2. Buchtitel
Leitfaden Molekulare Diagnostik
Kap. 5, Die Detektion von PCR Produkten, C.Tiemann
in: Thiemann, Cullen, Klein (2006) – Verlag Wiley-VCH
3. Posterpräsentationen
Comparison of PCR and electrospray-ionization mass spectrometry (ESI-MS) with
species-specific diagnostic algorithms during a biodefense external quality
assurance exercise. Bryan R. Thoma, Daniela Knoop, Andrea Hartmann, Carsten Tiemann, Lothar Zöller, Christin Hinz, Daniela Jacob, Roland Grunow and Sabine Schmoldt, DGHM Jahrestagung 2012
Evaluation of analytical sensitivity and specificity of the biothreat assay for clinical Bacillus anthracis diagnostics by the PLEX-ID™ System. M. Hanczaruk, B. Thoma, M. Antwerpen, S. Schmoldt, C. Tiemann,
D. Knoop, A. Hartmann, L. Zöller, G. Grass, VAAM Jahrestagung 2012
PlexID System: evaluation of analytical sensitivity and specificity of the biothreat assay for clinical Bacillus anthracis diagnostics. B.R. Thoma, M. Hanczaruk, M.H. Antwerpen, S. Schmold, D. Knoop, A. Hartmann, C. Tiemann , G. Grass. Medical Biodefence Conference 2012
Identification of orthopoxviruses by PCR/electrospray-ionization mass spectrometry. H. Meyer, L. Dobrzykowski, D. Knoop, A. Hartmann, B.R. Thoma, C. Tiemann , S. Schmold, Medical Biodefence Conference 2012
Rapid identification of infectious pathogens with PCR/electrospray-ionization TOF mass spectrometry. C. Tiemann , D. Knoop, A. Hartmann, C. Busse, European Meeting on Molecular Diagnostics, 2011
Improvement of a RNA isolation method for carotid endarterectomy samples embedded in cryo-medium. D. Knoop, C. Lanckohr, G. Theilmeier, C. Tiemann, European Meeting on Molecular Diagnostics 2009
Evaluation of the KingFisher Flex© for high throughput extraction of DNA and RNA from clinical samples.
A. Hartmann, D.Knoop, T.Zinn, C.Tiemann, European Meeting on Molecular Diagnostics 2009
Evaluation of testing cadaveric serum for HIV, HCV and HBV viraemia.
C Tiemann and D. Knoop, European Meeting on Molecular Diagnostics 2007
Performance Evaluation of a New Instrument for Genomic orViral DNA or RNA Extraction for HBV Viral Load Testing. C. Tiemann , D. Knoop , C. Dunn , S. Schaffer, European Meeting on Molecular Diagnostics 2007
Rapid Detection of Norovirus Genogroups By Automated RNA-Extraction and Real-time RT-PCR
C. Tiemann, A. Vogel, B. Dufaux, M. Zimmer, D. Münstermann,J.-R. Krone and H.-J. Hagedorn, European Meeting on Molecular Diagnostics 2005
Rapid and sensitive detection of PCR products in clinical diagnostics using the electrophoresis system Caliper 1000. C. Tiemann, A. Vogel, B. Dufaux, M. Zimmer, J.-R. Krone and H.-J. Hagedorn, European Meeting on Molecular Diagnostics 2003
Rapid Detection of Chlamydia trachomatis by Real-time PCR Using SYBR-Green I
C. Tiemann, A. Vogel, B. Dufaux, M. Zimmer, J.-R. Krone and H.-J. Hagedorn, European Meeting on Molecular Diagnostics 2001
4. Vorträge
Möglichkeiten und Grenzen automatisierter Nachweissysteme in der molekularen Diagnostik.
C. Tiemann, STI-Jubiläumskongress der DSTIG, Berlin, 2012
PlexID – zukünftige Möglichkeiten zur Erweiterung der Routinediagnostik beim Nachweis von Infektionserregern.
C. Tiemann, 10. Wissenschaftliches Abbott Symposium, 2012
Identification of orthopoxviruses by PCR/electrospray-ionization mass spectrometry.
H. Meyer, L. Dobrzykowski, D. Knoop, A. Hartmann, B.R. Thoma, C. Tiemann , S. Schmold,
Medical Biodefence Conference 2012
Rapid identification of infectious pathogens with PCR/electrospray-ionization TOF mass spectrometry.
C. Tiemann , D. Knoop, A. Hartmann, C. Busse
European Meeting on Molecular Diagnostics, 2011
Implementierung der PCR/ESI-TOF MS Technologie im Routinelabor
C. Tiemann, DGHM Tagung 2011
Rapid identification of mixed- and single bacterial and fungal infections with PCR coupled to electrospray-ionization TOF mass spectrometry.
C. Tiemann, D. Knoop, A. Hartmann, DGHM Tagung 2011
Molekulare Identifizierung von Pathogenen mittels PCR / ESI-TOF Massenspektrometrie
C. Tiemann, Regensburger Meeting für angewandte Molekulare Diagnostik (REMMDI), 2011
Identifizierung und Charakterisierung von Bakterien und Viren mittels PlexID – Evaluierungsbericht.
C.Tiemann, 7. Abbott Anwender-Forum Molekulare Diagnostik, 2011
Massenspektometrische Untersuchung von STR-Systemen mittels ESI-TOF-MS/PCR – am Beispiel des aktuellen GEDNAP-Ringversuches C. Tiemann, 31. Spurenworkshop der Deutschen Gesellschaft für Rechtsmedizin, 2011
RealTime High Risk HPV Diagnostik : erste Praxiserfahrung
C. Tiemann, 5. Abbott Anwender-Forum, 2009
Abbott Molecular Automation Advisory Board (Co-Chair)
C. Tiemann, Amsterdam-Meeting 2010
„Neue Grippe“ – H1N1
C. Tiemann, Rotary Club Herford, 2009
Abbott RealTime High Risk HPV vs HC“ (Digene)
C. Tiemann, Fortbildungstagung Klinische Zytologie, München, 2009
Chlamydien-Screening: erster Erfahrungsbericht
C. Tiemann, 4. Abbott Anwender-Forum, 2008
Gen-Profile und Identifizierungswahn – Möglichkeiten und moralische Grenzen
C. Tiemann, FoeBuD 2008