Amoeba
(Entamoeba histolytica/dispar)
Material: | Antibody demonstration: 1 ml serum (external service) Antigen demonstration: Stool (Labor Krone), several stool samples, 2-3 days apart, fresh body warm stool, where possible, for demonstration of vegetative forms (pathogen evidence, Labor Krone), PCR from stool or other material on request (External services, Bernhard-Nocht-Institut, Hamburg) |
Methods: |
Mikroskopie → Hellfeldmikroskopie Ligandenassays → Enzyme Linked Immunosorbent Assay (ELISA) Amplifikationsverfahren → Real-time-PCR | Reference range | IgG: < 9,0 - 11,0 index
IgM: < 2,9 - 3,1 index
PCR: negative |
Indication | Suspicion of intestinal amoebiasis (amoebic dysentery), suspicion of extra intestinal amoebiasis |
Please note | Entamoeba histolytica is the cause for amoebic dysentery. Usually common in warm countries, however can be brought into temperate climates. Infection happens by ingestion of amoebic cysts in contaminated food, occasionally in contaminated drinking water or bath water. Complications of amoebic dysentery are colon perforation, liver abscess, less commonly lung- or brain abscess. Entamoeba dispar: Pure commensal without pathogenic potential. Antibody demonstration is suitable for diagnostics of extra intestinal manifestation, not for exclusion of acute intestinal infection. Proof of an acute intestinal infection is confirmed by stool diagnostics. The antigen-test in the stool and direct antibody evidence in the stool does not allow differentiation between entamoeba histolytica and dispar. For this, a further test via PCR is necessary. Further information can be found here: |
External services | ja Medizinisches Labor Bremen |
Accredited | ja |
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